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Published Protocols

High throughput measurement of endophyte growth:
High-throughput 96-well assay to measure impact of polyphenolic compounds on fungal growth. dx.doi.org/10.17504/protocols.io.j8nlkwondl5r/v1
 
High throughput quantification of phenolic compounds:
Quantification of foliar polyphenolic concentration using a 96-well microtitre method. dx.doi.org/10.17504/protocols.io.81wgbmxovpko/v1
 
 

Protocols for fungal DNA extraction for genotyping via PCR:

DNA extraction from fungal mycelium using Extract-n-Amp dx.doi.org/10.17504/protocols.io.ga4bsgw

 

Protocols for fungal DNA extraction for genome sequencing:

Large Volume Fungal Genomic DNA Extraction Protocol for PacBio dx.doi.org/10.17504/protocols.io.qtjdwkn

Small volume fungal genomic DNA extraction protocol for Illumina genome V.1 dx.doi.org/10.17504/protocols.io.3w9gph6

 

Protocol for growing fungi on solid media for RNA or DNA extraction:

Inoculating and harvesting fungal isolates on cellophane overlay. dx.doi.org/10.17504/protocols.io.qtedwje

 
 

Protocols to extract total gDNA from plants for microbial studies:

DNA Extraction Protocol for Plant and Lichen Tissues Stored in CTAB dx.doi.org/10.17504/protocols.io.fs8bnhw

96 well DNA Extraction Protocol for Plant and Lichen Tissue Stored in CTAB dx.doi.org/10.17504/protocols.io.fscbnaw

 

Protocols for ITS/16S library preparation for Illumina Amplicon Sequencing:

Illumina MiSeq Dual-barcoded Two-step PCR Amplicon Sequencing Protocol dx.doi.org/10.17504/protocols.io.fs9bnh6

 

 

 

 

 

 


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